Vincent Lebrun
Zinc fingers and oxidative stress: Reactivity towards singlet oxygen and hypochlorous acidDoigts de zinc et stress oxydant : réactivité vis-à-vis de l’oxygène singulet et de l’acide hypochloreux
Published on 18 November 2014
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Thesis presented November 18, 2014
Abstract :
Zinc finger proteins are widely spread in the living world. They constitute a large superfamily, with a zinc site of general formula [ZnII(Cys)4-X(His)X] (x=0, 1 or 2) as a common feature. The majority of such sites plays a purely structural role, however, recent examples as Hsp33 and RsrA demonstrate they can also been used to detect oxidative stress. Given the occurrence of zinc finger proteins and their key roles, it is of high biological interest to identify factors controlling their reactivity and to understand why some ROS are able to oxidize them
in vivo, on the contrary to H2O2. In this project, we decided to focus on two major ROS of biological relevance: hypochlorous acid (HOCl) and singlet oxygen (1O2). By use of peptide model complexes, reproducing perfectly the structure of some archetypal zinc fingers, we investigated the reactivity of zinc fingers toward those ROS.
Keywords:
Oxidative stress, zinc fingers, singlet oxygen, hypochlorous acid, cystein
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